protein sequence analysis & classification Search Results


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trifluoroacetic acid tfa  (Chem Impex International)
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n n dimethylacetamide dma  (Chem Impex International)
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n n dimethyl formamide dmf  (Chem Impex International)
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triethylamine et3n  (Chem Impex International)
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triethylamine et3n - by Bioz Stars, 2026-08
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protein sequence analysis  (InterPro Inc)
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Protein Sequence Analysis, supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein sequence analysis and classification  (InterPro Inc)
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InterPro Inc protein sequence analysis and classification
Calcium sensitivity of <t>recombinant</t> <t>TcPDP</t> and <t>TbPDP.</t> A–D, purification of TcPDP (A) and TbPDP (C) recombinant proteins by immobilized metal affinity chromatography. Total lysate (Lys), flow through (FT) and eluted/desalted fractions (rPDP and TbPDP) were analyzed by SDS-PAGE on 10% gel stained with Coomassie Brilliant Blue. Molecular weight (MW) markers are shown on the left side of panels A and C. Recombinant TcPDP (B) and TbPDP (D) enzymatic activities were assayed at different Ca2+ concentrations. E, enzyme kinetics of TcPDP. Recombinant TcPDP phosphatase activity was assayed in buffer containing 50 mm Hepes, pH 7.0, 50 μm MgCl2, 2 mm DTT, 100 nm free Ca2+, 120 μg of BSA and different concentrations of TcPDH E1α synthetic phosphopeptide as substrate. Error bars are smaller than the symbols used for some data points. Kinetic parameters of recombinant TcPDP obtained from linear regression using Michaelis-Menten equation are shown in the plot. Values are mean ± S.D. of three independent experiments (B, D, and E).
Protein Sequence Analysis And Classification, supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein sequence analysis and classification - by Bioz Stars, 2026-08
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high sensitivity protein internal sequence analysis service  (APRO SCIENCE)
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Calcium sensitivity of <t>recombinant</t> <t>TcPDP</t> and <t>TbPDP.</t> A–D, purification of TcPDP (A) and TbPDP (C) recombinant proteins by immobilized metal affinity chromatography. Total lysate (Lys), flow through (FT) and eluted/desalted fractions (rPDP and TbPDP) were analyzed by SDS-PAGE on 10% gel stained with Coomassie Brilliant Blue. Molecular weight (MW) markers are shown on the left side of panels A and C. Recombinant TcPDP (B) and TbPDP (D) enzymatic activities were assayed at different Ca2+ concentrations. E, enzyme kinetics of TcPDP. Recombinant TcPDP phosphatase activity was assayed in buffer containing 50 mm Hepes, pH 7.0, 50 μm MgCl2, 2 mm DTT, 100 nm free Ca2+, 120 μg of BSA and different concentrations of TcPDH E1α synthetic phosphopeptide as substrate. Error bars are smaller than the symbols used for some data points. Kinetic parameters of recombinant TcPDP obtained from linear regression using Michaelis-Menten equation are shown in the plot. Values are mean ± S.D. of three independent experiments (B, D, and E).
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protein sequence analysis program for ms, paws  (Proteometrics LLC)
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Proteometrics LLC protein sequence analysis program for ms, paws
Calcium sensitivity of <t>recombinant</t> <t>TcPDP</t> and <t>TbPDP.</t> A–D, purification of TcPDP (A) and TbPDP (C) recombinant proteins by immobilized metal affinity chromatography. Total lysate (Lys), flow through (FT) and eluted/desalted fractions (rPDP and TbPDP) were analyzed by SDS-PAGE on 10% gel stained with Coomassie Brilliant Blue. Molecular weight (MW) markers are shown on the left side of panels A and C. Recombinant TcPDP (B) and TbPDP (D) enzymatic activities were assayed at different Ca2+ concentrations. E, enzyme kinetics of TcPDP. Recombinant TcPDP phosphatase activity was assayed in buffer containing 50 mm Hepes, pH 7.0, 50 μm MgCl2, 2 mm DTT, 100 nm free Ca2+, 120 μg of BSA and different concentrations of TcPDH E1α synthetic phosphopeptide as substrate. Error bars are smaller than the symbols used for some data points. Kinetic parameters of recombinant TcPDP obtained from linear regression using Michaelis-Menten equation are shown in the plot. Values are mean ± S.D. of three independent experiments (B, D, and E).
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omiga 2.0 dna and protein sequence analysis software  (PrimerDesign Inc)
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PrimerDesign Inc omiga 2.0 dna and protein sequence analysis software
Calcium sensitivity of <t>recombinant</t> <t>TcPDP</t> and <t>TbPDP.</t> A–D, purification of TcPDP (A) and TbPDP (C) recombinant proteins by immobilized metal affinity chromatography. Total lysate (Lys), flow through (FT) and eluted/desalted fractions (rPDP and TbPDP) were analyzed by SDS-PAGE on 10% gel stained with Coomassie Brilliant Blue. Molecular weight (MW) markers are shown on the left side of panels A and C. Recombinant TcPDP (B) and TbPDP (D) enzymatic activities were assayed at different Ca2+ concentrations. E, enzyme kinetics of TcPDP. Recombinant TcPDP phosphatase activity was assayed in buffer containing 50 mm Hepes, pH 7.0, 50 μm MgCl2, 2 mm DTT, 100 nm free Ca2+, 120 μg of BSA and different concentrations of TcPDH E1α synthetic phosphopeptide as substrate. Error bars are smaller than the symbols used for some data points. Kinetic parameters of recombinant TcPDP obtained from linear regression using Michaelis-Menten equation are shown in the plot. Values are mean ± S.D. of three independent experiments (B, D, and E).
Omiga 2.0 Dna And Protein Sequence Analysis Software, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paracoccidioides gel proteins sequence analysis  (Broad Institute Inc)
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Broad Institute Inc paracoccidioides gel proteins sequence analysis
Calcium sensitivity of <t>recombinant</t> <t>TcPDP</t> and <t>TbPDP.</t> A–D, purification of TcPDP (A) and TbPDP (C) recombinant proteins by immobilized metal affinity chromatography. Total lysate (Lys), flow through (FT) and eluted/desalted fractions (rPDP and TbPDP) were analyzed by SDS-PAGE on 10% gel stained with Coomassie Brilliant Blue. Molecular weight (MW) markers are shown on the left side of panels A and C. Recombinant TcPDP (B) and TbPDP (D) enzymatic activities were assayed at different Ca2+ concentrations. E, enzyme kinetics of TcPDP. Recombinant TcPDP phosphatase activity was assayed in buffer containing 50 mm Hepes, pH 7.0, 50 μm MgCl2, 2 mm DTT, 100 nm free Ca2+, 120 μg of BSA and different concentrations of TcPDH E1α synthetic phosphopeptide as substrate. Error bars are smaller than the symbols used for some data points. Kinetic parameters of recombinant TcPDP obtained from linear regression using Michaelis-Menten equation are shown in the plot. Values are mean ± S.D. of three independent experiments (B, D, and E).
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sequence similarity and protein family analysis  (Coreco Inc)
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Calcium sensitivity of <t>recombinant</t> <t>TcPDP</t> and <t>TbPDP.</t> A–D, purification of TcPDP (A) and TbPDP (C) recombinant proteins by immobilized metal affinity chromatography. Total lysate (Lys), flow through (FT) and eluted/desalted fractions (rPDP and TbPDP) were analyzed by SDS-PAGE on 10% gel stained with Coomassie Brilliant Blue. Molecular weight (MW) markers are shown on the left side of panels A and C. Recombinant TcPDP (B) and TbPDP (D) enzymatic activities were assayed at different Ca2+ concentrations. E, enzyme kinetics of TcPDP. Recombinant TcPDP phosphatase activity was assayed in buffer containing 50 mm Hepes, pH 7.0, 50 μm MgCl2, 2 mm DTT, 100 nm free Ca2+, 120 μg of BSA and different concentrations of TcPDH E1α synthetic phosphopeptide as substrate. Error bars are smaller than the symbols used for some data points. Kinetic parameters of recombinant TcPDP obtained from linear regression using Michaelis-Menten equation are shown in the plot. Values are mean ± S.D. of three independent experiments (B, D, and E).
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Image Search Results


Calcium sensitivity of recombinant TcPDP and TbPDP. A–D, purification of TcPDP (A) and TbPDP (C) recombinant proteins by immobilized metal affinity chromatography. Total lysate (Lys), flow through (FT) and eluted/desalted fractions (rPDP and TbPDP) were analyzed by SDS-PAGE on 10% gel stained with Coomassie Brilliant Blue. Molecular weight (MW) markers are shown on the left side of panels A and C. Recombinant TcPDP (B) and TbPDP (D) enzymatic activities were assayed at different Ca2+ concentrations. E, enzyme kinetics of TcPDP. Recombinant TcPDP phosphatase activity was assayed in buffer containing 50 mm Hepes, pH 7.0, 50 μm MgCl2, 2 mm DTT, 100 nm free Ca2+, 120 μg of BSA and different concentrations of TcPDH E1α synthetic phosphopeptide as substrate. Error bars are smaller than the symbols used for some data points. Kinetic parameters of recombinant TcPDP obtained from linear regression using Michaelis-Menten equation are shown in the plot. Values are mean ± S.D. of three independent experiments (B, D, and E).

Journal: The Journal of Biological Chemistry

Article Title: Calcium-sensitive pyruvate dehydrogenase phosphatase is required for energy metabolism, growth, differentiation, and infectivity of Trypanosoma cruzi

doi: 10.1074/jbc.RA118.004498

Figure Lengend Snippet: Calcium sensitivity of recombinant TcPDP and TbPDP. A–D, purification of TcPDP (A) and TbPDP (C) recombinant proteins by immobilized metal affinity chromatography. Total lysate (Lys), flow through (FT) and eluted/desalted fractions (rPDP and TbPDP) were analyzed by SDS-PAGE on 10% gel stained with Coomassie Brilliant Blue. Molecular weight (MW) markers are shown on the left side of panels A and C. Recombinant TcPDP (B) and TbPDP (D) enzymatic activities were assayed at different Ca2+ concentrations. E, enzyme kinetics of TcPDP. Recombinant TcPDP phosphatase activity was assayed in buffer containing 50 mm Hepes, pH 7.0, 50 μm MgCl2, 2 mm DTT, 100 nm free Ca2+, 120 μg of BSA and different concentrations of TcPDH E1α synthetic phosphopeptide as substrate. Error bars are smaller than the symbols used for some data points. Kinetic parameters of recombinant TcPDP obtained from linear regression using Michaelis-Menten equation are shown in the plot. Values are mean ± S.D. of three independent experiments (B, D, and E).

Article Snippet: TbPDP and TcPDP belong to the PP2C/PPM family, which is a group of Mg 2+ /Mn 2+ -dependent serine/threonine phosphatases essential for regulation of cell cycle and stress signaling pathways in different cell types (InterPro protein sequence analysis and classification) ( 33 ).

Techniques: Recombinant, Purification, Affinity Chromatography, SDS Page, Staining, Molecular Weight, Activity Assay, Phospho-proteomics